Identify the upstream region that contains the main enhancer and promoter region

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A gene expressed in long muscle of the mouse is identified, and the regulatory region upstream of the gene is isolated. Various segments of the upstream sequence are fused to the lacZ gene, and each fusion is assayed to determine how efficiently it transcribes the gene. In the accompanying diagram, the dark bars indicate the upstream segments that are present in each of six different fusion genes. The transcriptional efficiency of each fusion is measured against the control fusion, that is, the full-length upstream segment fused to the lacZ gene.

Part A
Identify the upstream region that contains the main enhancer.

Identify the upstream region that contains the main enhancer.

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Part B
Identify the upstream region containing the promoter.

Identify the upstream region containing the promoter.

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